Basic Information                  
Event GTS 40-3-2
OECD UI MON-Ø4Ø32-6
Trade Name Roundup Ready® Soybean
Species Glycine max (Soybean)
Introduced Trait Herbicide Tolerant
Transformation Method Microparticle bombardment
Transformation Vector PV-GMGT04
Developer Monsanto Company
Introduced Elements
All Known Sequence:
(1)
ref:    |    AJ308515    ||    AJ308514    ||    AB209952    |
Plant DNA CaMV 35S CTP4 Cp4 epsps nos Cp4 epsps trunc unknown Plant DNA
+ View Complete Sequence & Detailed Infomation
1CCCAAGTTCC TAAATCTTCA AGTTTTCTTG TTTTTGGATC TAAAAAACTG
51AAAAATTCAG AACCTTGTGC AAATTATTCA AACCCTTCAA TTTAACCGAT
101GCTAATGAGT TATTTTTGCA TGCTTTAATT TGTTTCTATC AAATGTTTAT
151TTTTTTTTAC TAGAAATAAC TTATTGCATT TCATTCAAAA TAAGATCATA
201CATACAGGTT AAAATAAACA TAGGGAACCC AAATGGAAAA GGAAGGTGGC
251TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC
301CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG
351TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT
401GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA
451AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGGACACGCT
Target Gene
cp4 epsps cassette
Promotor
P-35S-CTP4
enhanced CaMV 35S;chloroplast transit peptide from Petunia hybrida
Coding Region
Coding 5-enolpyruvylshikimate-3-phosphate synthase 
From Agrobacterium tumefaciens
Terminator
NOS 3'
A. tumefaciens nopaline synthase (nos) 3'-polyadenylation signal
    Molecular Characterization

As mentioned in the Monsanto's original petition, GTS40-3-2 contains a single insert, containing P-35S, CTP4, cp4 epsps, and T-nos. No other elements are found in the cotton genome.

Additional studies conducted by Monsanto showed that the insert of T-nos is complete. A 250 bp segment of the cp4 epsps, and a 534 bp rearrangement of the DNA are found adjacent to the 3' end of the T-nos. Besides, a 72 bp cp4 epsps inactive fragment is also found in the genome.

Nucleic Acid - Based Method
For Exogenous Gene:
StrategyMethod TypePrimer NameAmpliconProbe TypeValidation InfoStandard
ScreeningQualitativeP35S-cf3 / P35S-cr4123bpYesISO
HA-nos118-f / HA-nos118-r118bpYesISO
tNOS 2-5 / tNOS 2-3151bp
35s-1 / 35s-2195bpYesISO
35SFZMP1 / 35SFZMP2158bp
nosFZMP1 / nosFZMP2125bp
Quantitative35S-F / 35S-R83bpTaqManYesISO&National
sF / sR79bpTaqMan
P-0217 / P-0218121bpTaqMan
180-F / 180-R84bpTaqManYesNational
ChipHA-Eur118-f / HA-nos118-r118bp
T-35S_29-L / T-35S_29-R134bp
nos 3_30-L / nos 3_30-R138bp
CaMV35S_31-L / CaMV35S_31-R188bp
Gene-SpecificQualitativesttmf3a / sttmr2a145bp
QuantitativeRRS-F / RRS-R84bpTaqMan
Construct-SpecificQualitativeGM07 / GM08169bp
p35s-f2 / petu-r1172bpYesISO&National
SPA / SPB320bp
P-E35S for / RR rev125bp
QuantitativeRRS 01-5 / RRS 01-3121bpTaqManYesNational
RR-F / RR-RUnknownTaqMan
RR1-F / RR1-R74bpTaqMan
41_67-L / 41_67-R83bpTaqManYesISO
35s-f2 / petu-r1172bpTaqManYesISO
Chipp35s-f2 / petu-r1172bp
RR2075 For / NOSterN Rev210bpLDR-UA
RRS2075F / RRS2075R70bpMGB
Event-SpecificQualitativeRR04 / RR05180bp
41_39-L / 41_39-R151bp
41_40-L / 41_40-R177bp
35S2 / RRS359bp
p35s-f2 / petu-r1172bp
35G-F / 35G-R369bp
GTS40-3-2-F / GTS40-3-2-R370bpNational
Quantitative41_1-L / 41_1-R94bpTaqMan
RRS3JFor / RRS3JRev85bpTaqMan
41_3-L / 41_3-R119bpFRET
41_29-L / 41_29-R170bpScorpion
35SL / JUND199bp
35S4-JUNC / RRS3-JUNC148bpTaqMan
RRS SYB 101 F / RRS SYB 101 R101bpSYBR Green
RRS SYB 201 F / RRS SYB 201 R201bpSYBR Green
RRS SYB 301 F / RRS SYB 301 R301bpSYBR Green
RRS TaqL F / RRS TaqL R161bpTaqMan
RRS TaqR F / RRS TaqR R151bpTaqMan
RRS MGB L F / RRS MGB L R156bpMGB
RRS MGB J F / RRS MGB J R77bpMGB
RRS MGB R F / RRS MGB R R156bpMGB
RRS MB L F / RRS MB L R151bpMolecular Beacon
RRS MB J F / RRS MB J R77bpMolecular Beacon
RRS MB R F / RRS MB R R152bpMolecular Beacon
35S3 / RRS5123bpTaqMan
nos-junc-F1 / primer E189bpTaqMan
Chip41_56-L / 41_56-R187bp
41_95-L / 41_95-R256bp
RRS-AF / RRS-AR139bp
LAMPGTS-F3 / GTS-B3UnknownSYBR Green
*View All Possible Screen Method
*View All Possible Gene-Specific Method
For Endogenous Reference Gene:
lectin Acc. No: K00821 Copy No (haploid): 1
Protein - Based Method
Reported Method
Method Type Taget Gene Method Name Ring Trial Standard
ELISAcp4 epspsprEN ISO 21572 Method for RR soybeanValidatedISO
cp4 epspsELISA for RR soybean (Monsanto)
Commercial Kit
Method Type Taget Gene Product Name Producer
ELISA Kitcp4 epspsGMO Soya Test KitStrategic Diagnostics Inc
Strip Kitcp4 epspsQuickStix (TM) Strips for Roundup Ready® in SoybeansEnviroLogix, Inc.
cp4 epspsAgri-Screen CP4 Strip TestNeogen Corp.
cp4 epspsRoundup Ready® ImmunoStrip(TM)Agdia, Inc.
Certified Reference Materials
Cat. No. Name Average SD
ERM-BF410A ROUNDUP READY SOYA (blank) <0.3g/kg g/kg
ERM-BF410B ROUNDUP READY SOYA (level 1) 1.0g/kg ±0.5g/kg
ERM-BF410C ROUNDUP READY SOYA (level 2) 5.0g/kg ±1.0g/kg
ERM-BF410D ROUNDUP READY SOYA (level 3) 10.0g/kg ±1.6g/kg
ERM-BF410E ROUNDUP READY SOYA (level 4) 20.0g/kg ±2.6g/kg
ERM-BF410F ROUNDUP READY SOYA (level 5) 50.0g/kg ±5.3g/kg
*View All CRMs (The CRMs are provided by IRMM, AOCS, and Sigma)
Reference Molecules: pMuISL2pMuISL2
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